Introduction. The precise handling of micro/nano objects is essential for a wide range of applications in cellular biology1,2,3,4, microbiology5,6, chemistry7,8, and micro/nanotechnology9,10. Various manipulation techniques have been developed utilizing different mechanisms, including optical11,12,13,14,15,16, magnetic17,18, optic-electric19,20,21, and acoustic methods22,23,24,25,26,27,28. Within these techniques, optical tweezers stand out for their exceptional precision at the nanoscale and ...